Enzyme-linked Receptors

Written by: Jennifer Heyes from Manchester University,

 

Enzyme-linked receptor proteins either possess an in-built enzyme or associate with separate enzymes in the cytoplasm. These enzymes are activated upon ligand binding. They relay the extracellular signal to the nucleus by a sequence of interactions that eventually turn on specific transcription factors, altering gene expression in the cell.

 

Basic receptor structure

All enzyme-linked receptors share a few common features;

1. Ligand-binding domain

  • Extracellular to allow easy access for ligands.
  • Strong affinity for specific ligands - allows different ligands that bind to the same receptor to evoke particular cellular responses.

 

2. Transmembrane domain

  • Contains a series of hydrophobic amino acids.
  • Tethers the receptor to the cell membrane.

 

3. Cytosolic "active" enzyme domain

  • Either intrinsic to the receptor or tightly bound via the cytosolic domain.
  • The majority are kinases; they phosphorylate specific threonine, serine, and tyrosine amino acid residues (THR,S,TY = THIRSTY).

 

 

Signal relay

Within an organism, the processes of metabolism, growth, and differentiation must be tightly integrated for correct function. Cell signalling essentially connects and lubricates these occurances to ensure that progress runs smoothly.

Doesn't it seems quite handy therefore, that "lubricating" is an easy way to remember the mechanism of signal relay by enzyme linked receptors...?!

 

                                                            Ligands are released into the extracellular space

                                                            Upon

                                                            Binding

                                                 to their Receptor

                                                specific Interactions cause a 

                                                            Conformational change within the catalytic domain,

                                                      this Activates the connected enzyme,

                     phosphorylation generates Tethering sites

                                                   where Intracellular effector proteins bind

         these further relay the signal to the Nucleus

                           resulting in changes in Gene expression

 

 

Enzyme-linked receptor classes

There are three main types of enzyme-linked receptors:

 

1. Receptor serine-threonine kinases e.g. transforming growth factor-beta (TGFB) receptors. 

2. Receptor tyrosine kinases (RTKs) e.g. growth factor receptors.

3. Tyrosine-kinase-associated receptors e.g. cytokine receptors. 

 

The figure below compares each aspect of the three signalling pathways which are explained in more detail in the following sections.

comparison of enzyme-linked receptors

1. Receptor serine/threonine kinases

There are two types of serine/threonine kinase receptors, both of which contain an intracellular kinase domain. They are each dimeric proteins, so an active receptor complex is made up of four receptors.

 

1. Type I receptors

  • Inactive unless in complex with type II receptors.
  • Do not interact with ligand dimers.
  • Contain conserved sequences of serine and threonine residues near to their kinase domains.

 

2. Type II receptors

  • Constitutively active kinase domains (even in the absence of the bound ligand).
  • Able to phosphorylate and activate the type I receptor.

 

Type I receptors are kept inactive by a portion of its cytosolic domain that blocks its kinase activity.

TGFB/bone morphogenetic protein (BMP)/activin ligands bind as dimers to Type II receptors.

Type II receptors then bind to, and phosphorylate, Type I receptors. This removes the inhibition of Type I kinase activity. 

Type I receptors then phosphorylate Smad transcription factors, allowing them to dimerise and enter the nucleus to repress or activate target gene expression.

For an animation of this pathway see: http://www.youtube.com/watch?v=QoJBIfM0bmU

2. Receptor tyrosine kinases (RTKs)

RTK ligands, such as fibroblast growth factor (FGF), epidermal growth factor (EGF), nerve growth factor (NGF) etc. bind as dimers.

 

1. Ligand binding to RTK monomers results in dimer formation.

2. Receptors possess an intracellular tyrosine kinase domain. Within the dimer the conformation is changed, locking the kinase into an active state.

3. The kinase of one receptor then phosphorylates a tyrosine residue contained in the "activation lip" of the second receptor.

This forces the activation lip out of the kinase active site, allowing ATP bind and resulting in enhanced kinase activity.

This induces phosphorylation at further tyrosine residues.

4. Phosphotyrosine is a conserved "docking site" for many intracellular signal transduction proteins that contain SH2 domains (see further down the page for more information).

 

For animations and summaries of this pathway see: 

http://www.wiley.com/college/fob/quiz/quiz21/21-15.html

http://www.youtube.com/watch?v=_Z1uu0rXFDc&feature=related

 

RTK pathway

3. Tyrosine-kinase-associated receptors

Cytokines are the main ligands that signal through tyrosine kinase-associated receptors. 

Like RTKs and RS/TKs these receptors activate a cascade of phosphorylation BUT they do not possess a tyrosine kinase domain.

The intracellular side of each receptor is bound to a cytosolic tyrosine kinase protein.

 

1. Cytokines bind simultaneously to two receptor monomers.

2. This brings the two associated kinases closer together.

3. One kinase phosphorylates the other kinase in an area called the "activation lip" (similar to RTK activation).

The activation lip moves out of the active site and binds ATP therefore enhancing kinase activity.

4. The enhanced kinase phosphorylates more tyrosine residues on the intracellular portion of the receptor.

5. Phosphotyrosines serve as "docking sites" for SH2 domain-containing proteins (see section further down the page for more information)

 

tyrosine-kinase-associated receptor activation

For an animation of the tyrosine-kinase-associated receptor pathway janus kinase (JAK)-signal transducer and activator of transcription (STAT) see: 

http://www.youtube.com/watch?v=G4K6IQZGHJc

 

SH2 domains

Src homology 2 (SH2) domains bind to phosphotyrosine residues, along with other similar domains such as the phosphotyrosine-binding (PTB) domain.

 

These domains are found in a HUGE variety of proteins....

 

 - enzymes such as kinases, phosphatases

 - scaffolding proteins that promote the formation of protein complexes

 - adaptors that act alongside receptors to promote function

 - regulators that inhibit or dampen signalling by the receptor

 

...all of which play a role in regulating "cross-talk" between different signalling pathways.

 

It's important to note that SH2 domain-containing proteins are very specific; they do not simply bind to any old phosphotyrosine residue, but take into account other amino acid residues around this site.

 

References/Further Reading

Alberts, B et al (2008) "Signaling through enzyme linked receptors" in Molecular Biology of the Cell, 5th Edition. New York, Garland Science

Lodish, H et al (2008) "Cell Signaling I and II" in Molecular Cell Biology, 6th Edition. New York, W. H. Freeman and Company